|
KMID : 1098420070150030210
|
|
Korean Journal of Medicinal Crop Science
2007 Volume.15 No. 3 p.210 ~ p.214
|
|
|
Effect of Plant Growth Regulators, Media and Celling Agents on In Vitro Microtuber Production of Pinellia ternata Breit
|
|
Kim Yong-kyung
Lee Ji-Yeon
Kim Eung-Hwi
Cho Dong-Ha
Park Sang-Un
|
|
|
Abstract
|
|
|
|
The study was carried out to establish in vitro microtuber production from leaf and petiole explant cultures of Pinellia ternata. Culture conditions were optimized by investigating the effect of plant growth regulators, different media, and gelling agents on the efficiency of microtuber indurtion. Among the different combinations of plant growth regulators tested, the combination of 0.1 mg/l 2,4-Dichlorophenoxyacetic (2,4-D) and 0.5 mg/l 6-benzylaminopurine (BAP) showed the highest yield fer microtuber production from leaf (3.9) and petiole (4.7) explant culture on MS medium far 6 weeks. SH(Schenk and Hildebrandt) medium was the most effective medium for microtuber induction and the half strength SH medium was better than SH medium for microtuber production from both leaf and petiole culture. Gelrite was better than agar in the formation of microtubers and 4% Gelrite showed the highest number of microtubers per explant frome leaf (5.9) and petiole (7.8) culture. Germination rate of microtubers after cold storaged for one months long was 86% from in vitro culture and 43% from autoclaved soil.
|
|
|
KEYWORD
|
|
|
gelling agent, media, microtuber, Pinellia ternata Breit., plant growth regulators
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|